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Northern Blot Analysis of mRNA From Mammalian Polyribosomes Protocol - Details

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Protocols » Listing Details
ID:3263
Title: Northern Blot Analysis of mRNA From Mammalian Polyribosomes Protocol - http://www.natureprotocols.com/2006/09/15/northern_blot_analysis_of_mrna.php
Description:The protocol consists of a method for the generation of cytoplasmic extracts from mammalian cells (in this case, 293T cells) without the disruption of polyribosomes, the separation of ribosomal components and polyribosomes by sucrose gradient centrifugation, the isolation of mRNA from these fractions, and detection of mRNA by Northern blot analysis.
Category:Nucleic Acid Detection Protocols: Nucleic Acid Blotting Protocols
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Date Added:April 08, 2008 12:51:04 PM
Number Hits:40
 

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