Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Restriction Endonuclease Digestion of DNA in Agarose Plugs Protocol - Details

Link Details 

Protocols » Listing Details
ID:3260
Title: Restriction Endonuclease Digestion of DNA in Agarose Plugs Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4031
Description:Protocol for restriction endonuclease digestion of DNA in agarose plugs. Genomic DNA isolated from mammalian, yeast, or bacterial cells can be digested with restriction endonucleases by incubating agarose plugs containing the DNA in the presence of the desired enzyme.  After digestion, the DNA can be fractionated by pulsed-field gel electrophoresis and either isolated from the gel or analyzed by Southern Hybridization.
Category:Nucleic Acid Electrophoresis Protocols: Pulsed Field Gel Electrophoresis Protocols
Link Owner:
Date Added:April 08, 2008 12:32:45 PM
Number Hits:41
 

Protocols Tags

  • adaptors Protocols
  • adult Protocols
  • amplification Protocols
  • analgesia Protocols
  • analysis Protocols
  • assay Protocols
  • cdna Protocols
  • cells Protocols
  • center Protocols
  • chromatin Protocols
  • confocal Protocols
  • culture Protocols
  • cytokine Protocols
  • describes Protocols
  • detection Protocols
  • digestions Protocols
  • embryos Protocols
  • flow Protocols
  • fluorescent Protocols
  • genotyping Protocols
  • hla Protocols
  • hybridization Protocols
  • includes Protocols
  • isolation Protocols
  • ivcca Protocols
  • linkers Protocols
  • live Protocols
  • microscope Protocols
  • mirnas Protocols
  • mouse Protocols
  • muscle Protocols
  • post Protocols
  • preparation Protocols
  • protein Protocols
  • resting Protocols
  • rna Protocols
  • signal Protocols
  • signaling Protocols
  • single Protocols
  • situ Protocols
  • skeletal Protocols
  • sscp Protocols
  • standard Protocols
  • tissue Protocols
  • tsa Protocols
  • two Protocols
  • tyramide Protocols
  • visualizing Protocols
  • yeast Protocols
  • zygotes Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum