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Purification of Synthetic Oligonucleotides by Polyacrylamide Gel Electrophoresis Protocol - Details

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Protocols » Listing Details
ID:3240
Title: Purification of Synthetic Oligonucleotides by Polyacrylamide Gel Electrophoresis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4063
Description:Protocol for purification of synthetic oligonucleotides by polyacrylamide gel electrophoresis. As a rule of thumb, oligonucleotides >25 nucleotides should be purified by polyacrylamide gel electrophoresis, as should oligonucleotides of any length that yield anomalous results.  After electrophoresis, the oligonucleotide is eluted from the gel and concentrated by reversed-phase chromatography on Sep-Pak C18 columns.
Category:Nucleic Acid Electrophoresis Protocols: DNA Electrophoresis Protocols: DNA Electrophoresis in Polyacrylamide Gels Protocols
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Date Added:April 08, 2008 12:01:58 PM
Number Hits:6
 

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