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Mapping Protein-binding Sites on DNA by Dnase I Footprinting Protocol - Details

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Protocols » Listing Details
ID:3209
Title: Mapping Protein-binding Sites on DNA by Dnase I Footprinting Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3947
Description:Dnase I is used to fragment a radiolabeled target DNA in the presence and absence of a nuclear extract.  A "footprint" is generated when a protein binds to the target and protects a specific segment of DNA from the nucleolytic activity of Dnase I. By comparing the electrophoretic mobility of the Dnase I cleavage products to those of a sequence ladder derived from the same DNA fragment, the position(s) of the DNA sequences recognized by DNA-binding proteins can be determined.
Category:DNA Protein Interactions Protocols: Dnase Footprinting Assay Protocols
Link Owner:
Date Added:March 22, 2008 03:03:14 AM
Number Hits:105
 

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