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Preparation of siRNAs from Drosophila Embryo Extracts Protocol - Details

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Protocols » Listing Details
ID:3194
Title: Preparation of siRNAs from Drosophila Embryo Extracts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4325
Description:siRNAs produced upon the addition of dsRNA to Drosophila embryo extract are enriched in a micrococcus-nuclease-resistant fraction.  After proteinase K treatment and dephosphorylation with calf intestinal phosphatase, these siRNAs mediate efficient RNAi in vitro.
Category:RNAi Technology Protocols: RNAi Drosophila Protocols
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Date Added:March 19, 2008 10:01:05 AM
Number Hits:18
 

 

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