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Delivery of dsRNA into Drosophila Embryos by Gene Gun Protocol - Details

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Protocols » Listing Details
ID:3192
Title: Delivery of dsRNA into Drosophila Embryos by Gene Gun Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4517
Description:Protocol describes how subcellular-sized particles are accelerated to high velocity to carry double-stranded RNA (dsRNA) into Drosophila embryos.  The major advantage of this procedure over microinjection (Microinjection of dsRNA into Drosophila Embryos) is that particle bombardment is easier and faster to perform.  In addition, the mechanical trauma received is far less than by microinjection, allowing better survival of embryos and fewer phenotypic artifacts.
Category:RNAi Technology Protocols: RNAi Drosophila Protocols
Link Owner:
Date Added:March 19, 2008 09:59:16 AM
Number Hits:29
 

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