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Hybridization and Detection Using the HC ExpressArray Kit Protocol - Details

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Protocols » Listing Details
ID:3115
Title: Hybridization and Detection Using the HC ExpressArray Kit Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/14/pdb.prot4122
Description:Protocol describes the direct detection of RNA on DNA microarrays using Hybrid Capture (HC) technology and the HC ExpressArray Kit developed by Diagene.  The kit uses a proprietary antibody that binds specifically to RNA:DNA hybrids and a second, fluorescently labeled, antibody that detects the primary antibody.  Total RNA is applied directly to a glass-spotted DNA microarray, and stable RNA:DNA hybrids are visualized via a Cy3-labeled secondary antibody.
Category:Microarray Protocols: Microarray Hybridization Protocols
Link Owner:
Date Added:March 05, 2008 07:46:58 PM
Number Hits:20
 

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