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Concentration of Oligo DNA by Ethanol Precipitation Protocol - Details

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Protocols » Listing Details
ID:3107
Title: Concentration of Oligo DNA by Ethanol Precipitation Protocol - http://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA/precip.html
Description:Concentration of DNA by Ethanol Precipitation Protocol. Adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma. Usually 2.5 - 3 volumes of  ethanol and/or acetate solution is added to the DNA in a microcentrifuge tube. This is then put into an ice-water bath for at least 10 minutes. The precipitation is performed by incubation at -20C overnight.
Category:Oligonucleotide Protocols: Oligonucleotide Purification Protocols
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Date Added:March 05, 2008 04:49:28 AM
Number Hits:189
 

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