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UV Absorbance 280 nm Protein Determination - Details

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Protocols » Listing Details
ID:3104
Title: UV Absorbance 280 nm Protein Determination - http://wolfson.huji.ac.il/purification/Protocols/OD280.html
Description:UV Absorbance 280 nm Protein Determination. Simple and quick method to accurately quantitate total protein in purified material or approximately quantitate total protein in crude lysates or partial purified material. Quantitation of the amount of protein in a solution is possible in a simple spectrometer.  Absorption of radiation in the near UV by proteins depends on the Tyr and Trp content (and to a very small extent on the amount of Phe and disulfide bonds).  Dr. Mario Lebendiker
Category:Protein Protocols: Protein Quantitation Concentration Protocols
Link Owner:
Date Added:March 05, 2008 02:51:45 AM
Number Hits:297
 

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