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Phosphatase Treatment of Linearized Vector Protocol - Details

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Protocols » Listing Details
ID:3017
Title: Phosphatase Treatment of Linearized Vector Protocol - http://openwetware.org/wiki/Phosphatase_treatment_of_linearized_vector
Description:To minimize self-ligated vector in your transformation, treat your linearized vector with a phosphatase to remove the 5' phosphates necessary for ligation. This should improve the percentage of colonies with inserts.
Category:Cloning Protocols: Vector Protocols
Link Owner:
Date Added:February 21, 2008 10:58:49 PM
Number Hits:77
 

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