Phosphatase Treatment of Linearized Vector Protocol - Details

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ID:	3017
Title:	Phosphatase Treatment of Linearized Vector Protocol - http://openwetware.org/wiki/Phosphatase_treatment_of_linearized_vector
Description:	To minimize self-ligated vector in your transformation, treat your linearized vector with a phosphatase to remove the 5' phosphates necessary for ligation. This should improve the percentage of colonies with inserts.
Category:	Cloning Protocols: Vector Protocols
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Date Added:	February 21, 2008 10:58:49 PM
Number Hits:	141