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Directional Cloning into Plasmid Vectors Protocol - Details

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Protocols » Listing Details
ID:3014
Title: Directional Cloning into Plasmid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3919
Description:Directional cloning requires that the plasmid vector be cleaved with two restriction enzymes that generate incompatible termini and that the fragment of DNA to be cloned carries termini that are compatible with those of the doubly cleaved vector.
Category:Cloning Protocols: Vector Protocols
Link Owner:
Date Added:February 21, 2008 10:53:39 PM
Number Hits:265
 

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