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Ligation Optimization Protocol - Details

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Protocols » Listing Details
ID:3003
Title: Ligation Optimization Protocol - http://www.fhcrc.org/science/labs/fero/Protocols/Ligation.html
Description:Protocol can be used to optimize ligation conditions for difficult to clone (e.g. very large) fragments. The principle is to independently characterize the ligation kinetics of the vector and insert DNA fragments and then to combine them in optimal ratios.
Category:Cloning Protocols
Link Owner:
Date Added:February 21, 2008 09:22:34 PM
Number Hits:104
 

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