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In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI - Details

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Protocols » Listing Details
ID:2993
Title: In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3813
Description:Protocol for in vitro mutagenesis using double-stranded DNA templates. Two oligonucleotides are used to prime DNA synthesis catalyzed by a high-fidelity thermostable polymerase on a denatured plasmid template. The two oligonucleotides both contain the desired mutation and occupy the same starting and ending positions on opposite strands of the plasmid DNA.
Category:Cloning Protocols: Mutagenesis Protocols
Link Owner:
Date Added:February 21, 2008 09:04:47 PM
Number Hits:77
 

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