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Clone Genes From a Phage Library Protocol - Details

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Protocols » Listing Details
ID:2983
Title: Clone Genes From a Phage Library Protocol - http://www.ucsf.edu/micro/faculty/Johnson/protocols/protocol1.html
Description:Protocol for cloning genes from a phage library. Includes: Titer and plate out phage; Lift plaques onto filters and prepare them for screening; Make a probe; Hybridize the probe to the filters; Wash the filters and expose to film; Purify putative plaques; Excise plasmid from the desired phage.
Category:DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols
Link Owner:
Date Added:February 12, 2008 02:42:55 PM
Number Hits:72
 

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