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Generation of a Library of Randomly Overlapping DNA Inserts Protocol - Details

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Protocols » Listing Details
ID:2975
Title: Generation of a Library of Randomly Overlapping DNA Inserts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4081
Description:Shotgun sequencing of a large segment of DNA involves random fragmentation of the target region into smaller segments that are subsequently cloned into a bacteriophage M13 vector. The goal is to create a library of overlapping clones that provide at least fivefold coverage over the entire length of the target fragment.
Category:DNA Protocols: cDNA Library Protocols: cDNA Library Construction Protocols
Link Owner:
Date Added:February 12, 2008 01:35:04 PM
Number Hits:22
 

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