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Construction of Genomic DNA Libraries in Cosmid Vectors Protocol - Details

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Protocols » Listing Details
ID:2972
Title: Construction of Genomic DNA Libraries in Cosmid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4000
Description:Protocol describes how to construct a library of 35-45-kb fragments of genomic DNA in the double cos site cosmid vector, SuperCos-1. The steps include: Linearization and dephosphorylation of SuperCos-1 DNA; Partial digestion of high-molecular-weight DNA with MboI; Dephosphorylation of high-molecular-weight genomic DNA; Ligation of cosmid arms to genomic DNA: Packaging and plating recombinants; Isolation and analysis of recombinant cosmids: Validation of the library.
Category:DNA Protocols: Cosmid Library Protocols
Link Owner:
Date Added:February 12, 2008 01:28:53 PM
Number Hits:110
 

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