Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Detection of Ubiquitylated Proteins in Yeast Protocol - Details

Link Details 

Protocols » Listing Details
ID:2892
Title: Detection of Ubiquitylated Proteins in Yeast Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4615
Description:This assay is performed to detect ubiquitylated proteins in yeast. Yeast that have been transformed with a vector expressing polyhistidine-tagged ubiquitin (Ub) under the control of a copper-inducible promoter are grown, induced with copper, and harvested. Total ubiquitylated proteins are then recovered by nickel-affinity chromatography, and specific proteins are detected by Western blotting.
Category:Yeast Protocols: Yeast Protein Protocols
Link Owner:
Date Added:February 10, 2008 01:32:24 PM
Number Hits:39
 

Protocols Tags

  • adherent Protocols
  • affect Protocols
  • all Protocols
  • animal Protocols
  • antibody Protocols
  • antisense Protocols
  • atpase Protocols
  • blocking Protocols
  • calcium Protocols
  • cell Protocols
  • cells Protocols
  • competent Protocols
  • cos Protocols
  • counterstaining Protocols
  • coverslip Protocols
  • density Protocols
  • detection Protocols
  • electroporation Protocols
  • fibroblasts Protocols
  • filters Protocols
  • fixation Protocols
  • immunofluorescence Protocols
  • include Protocols
  • involves Protocols
  • ldl Protocols
  • may Protocols
  • membrane Protocols
  • microscopy Protocols
  • mouse Protocols
  • pdf Protocols
  • phage Protocols
  • plaques Protocols
  • plasma Protocols
  • preparation Protocols
  • protein Protocols
  • proteins Protocols
  • raw Protocols
  • requires Protocols
  • silver Protocols
  • specific Protocols
  • staining Protocols
  • test Protocols
  • that Protocols
  • these Protocols
  • tissue Protocols
  • titer Protocols
  • transfection Protocols
  • transgenic Protocols
  • yeast Protocols
  • 0 Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum