Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Identification of End Clones in YAC Subclone Libraries Protocol - Details

Link Details 

Protocols » Listing Details
ID:2882
Title: Identification of End Clones in YAC Subclone Libraries Protocol - http://humgen.wustl.edu/hdk_lab_manual/yeast/yeast10.html
Description:To identify the YAC subclones containing both a human insert and a portion of either the left or right arm of the pYAC4 vector. Identification of these clones is necessary in order to do YAC chromosome walking, and is also useful in the determination of whether a particular YAC clone has a contiguous human insert or whether a co-cloning event has occurred. Vector arm sequences are identified using pBR322 fragments from a BamHI-PvuII double digest.
Category:Yeast Protocols: Yeast Nucleic Acid Protocols: Yeast PCR Protocols
Link Owner:
Date Added:February 10, 2008 01:07:19 PM
Number Hits:7
 

Protocols Tags

  • analysis Protocols
  • ascospores Protocols
  • assay Protocols
  • band Protocols
  • basic Protocols
  • cell Protocols
  • cells Protocols
  • collected Protocols
  • containing Protocols
  • culture Protocols
  • defrosting Protocols
  • denaturing Protocols
  • determination Protocols
  • eukaryotic Protocols
  • fractionation Protocols
  • freezing Protocols
  • frozen Protocols
  • gel Protocols
  • glycosphingolipid Protocols
  • how Protocols
  • includes Protocols
  • information Protocols
  • iridium Protocols
  • isolating Protocols
  • isolation Protocols
  • liquid Protocols
  • long Protocols
  • make Protocols
  • medium Protocols
  • methods Protocols
  • needles Protocols
  • nucleic Protocols
  • pcr Protocols
  • platinum Protocols
  • polymerase Protocols
  • preparation Protocols
  • procedures Protocols
  • quality Protocols
  • reagents Protocols
  • rnai Protocols
  • safety Protocols
  • sage Protocols
  • shift Protocols
  • tapered Protocols
  • that Protocols
  • thawing Protocols
  • them Protocols
  • tips Protocols
  • tissue Protocols
  • tissues Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum