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Growth of S. cerevisiae and Preparation of DNA Protocol - Details

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Protocols » Listing Details
ID:2837
Title: Growth of S. cerevisiae and Preparation of DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3298
Description:Protocol describes methods for isolation of DNA from a strain of S. cerevisiae carrying a recombinant YAC. Because the linear YAC DNAs are sensitive to shearing forces, pipettes with wide-bore tips should be used to transfer DNAs. The method is suitable for preparing DNA that will be used for agarose gel electrophoresis, Southern blotting, subcloning, genomic library construction, PCR, or other methods that do not require intact high-molecular-weight DNA.
Category:Yeast Protocols: Yeast Genetics Protocols: YACs Protocols
Link Owner:
Date Added:February 03, 2008 11:34:49 PM
Number Hits:26
 

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