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Assay for Phage Containing the Beta-galactosidase Gene Protocol - Details

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Protocols » Listing Details
ID:2826
Title: Assay for Phage Containing the Beta-galactosidase Gene Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda7.html
Description:This assay is used when working with phage vectors carrying the beta-galactosidase gene (often used for immunological screening). If the cloning event disrupts a normally functional copy of the gene in the vector the resulting plaques would appear clear in the assay. If the phages contain a functional beta-galactosidase gene they will form blue rings around their plaques. Any strain which is not an overproducer of beta- galactosidase will work as indicator host bacteria.
Category:Yeast Protocols: Yeast Genetics Protocols: B-Galactosidase Assay Protocols
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Date Added:February 03, 2008 11:19:29 PM
Number Hits:23
 

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