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Induction of Conjugation in Tetrahymena Protocol - Details

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Protocols » Listing Details
ID:2819
Title: Induction of Conjugation in Tetrahymena Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4499
Description:Mature Tetrahymena cells of opposite mating types are starved under appropriate salt conditions. The mating types are then combined to costimulate through cell-cell interaction. Loose pairs and then firm, irreversible pairs of cells of opposite mating types form. This method consistently results in a high percentage of pairing (usually greater than 80%) and good synchrony.
Category:Tetrahymena Protozoa Protocols
Link Owner:
Date Added:February 03, 2008 01:10:31 AM
Number Hits:8
 

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