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Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol - Details

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Protocols » Listing Details
ID:2800
Title: Antibody Addition to Drosophila Specimens and Detection Using Enzyme-Linked Reagents Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4526
Description:Enzyme-linked reagents give excellent sensitivity and use a simple light microscope for detection. A range of enzymes is available, but for staining in situ, horseradish peroxidase will suit most needs. Diaminobenzidine (DAB) is one of the most sensitive substrates for horseradish peroxidase. It yields an intense brown product that is insoluble in both water and alcohol. It can be made more sensitive by adding metal salts such as cobalt or nickel to the substrate solution.
Category:Drosophila Protocols
Link Owner:
Date Added:February 02, 2008 11:57:59 PM
Number Hits:37
 

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