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Preparing Late Whole-Mount Drosophila Embryos for Immunostaining Protocol - Details

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Protocols » Listing Details
ID:2766
Title: Preparing Late Whole-Mount Drosophila Embryos for Immunostaining Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4525
Description:Early and late embryos are treated with a mixture of organic solvents, formaldehyde, and alcohols. The cuticles of late-stage embryos (17-22 hours or until hatching) are usually opened by sonication, as described here. Tissues from later stages of development are normally dissected by hand and then fixed and stained in a standard paraformaldehyde/detergent combination.
Category:Drosophila Protocols: Drosophila Staining Protocols
Link Owner:
Date Added:February 02, 2008 01:23:44 AM
Number Hits:28
 

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