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Production of dsRNA for RNAi in Avian Embryos Protocol - Details

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Protocols » Listing Details
ID:2759
Title: Production of dsRNA for RNAi in Avian Embryos Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4503
Description:Protocol describes the production of double-stranded RNA (dsRNA) from fragments of cDNAs of candidate genes. The cDNA fragments must be cloned in plasmids with a flanking SP6 and T7 promoter (e.g., pSP72 or pCRII). The plasmid is linearized and sense and antisense RNAs are produced separately by in vitro transcription. After purification, the RNA strands are annealed to yield a dsRNA molecule suitable for RNAi in avian embryos.
Category:Avian Bird Protocols
Link Owner:
Date Added:February 01, 2008 11:28:21 PM
Number Hits:15
 

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