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Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods Protocol - Details

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Protocols » Listing Details
ID:2704
Title: Electroporating DNA into Embryonic Stem (ES) Cells and Selection Methods Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4409
Description:Protocol describes a method for electroporating DNA into ES cells, as well as selection methods. Pilot studies should be performed to optimize the conditions for each DNA construct. The selection method described here is one of the most complex. It involves targeting constructs in which the bacterial neomycin-resistance gene disrupts the coding sequence of the mouse gene.
Category:Mouse Protocols: Mouse Genetics Protocols
Link Owner:
Date Added:January 29, 2008 06:26:19 PM
Number Hits:41
 

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