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Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos Protocol - Details

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Protocols » Listing Details
ID:2654
Title: Assembling Aggregates between Embryonic Stem (ES) Cells and Tetraploid Embryos Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4426
Description:Protocol describes a method for producing ES cell-tetraploid embryo chimeras. It requires the timed combination of four-cell-stage tetraploid embryo production and the procedure for ES cell-diploid embryo aggregation in which diploid embryos are replaced with tetraploid embryos. The resulting chimeras can be used to analyze the embryonic versus extraembryonic phenotype of a mutation.
Category:Mouse Protocols: Assembling Chimeras Mouse Protocols
Link Owner:
Date Added:January 24, 2008 01:36:45 PM
Number Hits:3
 

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