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Expression of Cloned Genes in E. coli Using the Bacteriophage T7 Promoter Protocol - Details

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Protocols » Listing Details
ID:2638
Title: Expression of Cloned Genes in E. coli Using the Bacteriophage T7 Promoter Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3597
Description:Protocol for the expression of cloned genes in E. coli using the bacteriophage T7 promoter. Protocol describes how (1) to clone cloned sequences encoding open reading frames in plasmids carrying bacteriophage T7 promoters, (2) to optimize expression of target proteins in transformants carrying these recombinants, and (3) to scale-up production of foreign proteins.
Category:E Coli Protocols: E coli Protein Protocols
Link Owner:
Date Added:January 24, 2008 11:52:36 AM
Number Hits:113
 

 

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