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Louise- "How did you get here?" Johnny- "Well, basically, there was this little dot, right? And the dot went bang and the bang expanded. Energy formed into matter, matter cooled, matter lived, the amoeba to fish, to fish to fowl, to fowl to frog, to frog to mammal, the mammal to monkey, to monkey to man, amo amas amat, quid pro quo, memento mori, ad infinitum, sprinkle on a little bit of grated cheese and leave under the grill till Doomsday." ~From the movie Naked
Link Details
| ID: | 2627 |
| Title: | Preparation of Worm Extracts by Sonication Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Cyto_extract_sonicat_v2.htm |
| Description: | Protocol was designed to rapidly generate small scale cytosolic extracts of C. elegans for Western or IP (has not been tested for RNA work). The protocol works well for between 50 to 5000 worms and has not been extensively tested on larger a scale, though it should work. Includes: Collection; Sonication; Clearing lysate; Immunoprecipitation. |
| Category: | C. elegans Protocols |
| Link Owner: | |
| Date Added: | January 24, 2008 11:21:19 AM |
| Number Hits: | 25 |