Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Link Details
| ID: | 2608 |
| Title: | C. elegans RNAi Protocol - http://www.zoology.ubc.ca/~alorch/rnai-protocol.htm |
| Description: | Protocol for C. elegans RNAi. Includes: Transformation of competent cells; Blunt-end ligation; Preparation of competent cells; Dephosphorylation of linear plasmid DNA; Restriction Digest: EcoRV; Insert amplification from gDNA; Gel purification: QiaQuick gel purification kit; Mini-prep; Transformant Screening; Bacterial preparation and induction; Preparation of worms for RNAi feeding. |
| Category: | C. elegans Protocols: C. elegans Genetics Protocols |
| Link Owner: | |
| Date Added: | January 24, 2008 07:30:32 AM |
| Number Hits: | 36 |
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