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Precision Engineering of Plant Gene Loci by Homologous Recombination Cloning in Escherichia Coli - Details

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Protocols » Listing Details
ID:2539
Title: Precision Engineering of Plant Gene Loci by Homologous Recombination Cloning in Escherichia Coli - http://www.plantmethods.com/content/1/1/6
Description:Protocol for precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli. Includes: Key steps in the EL250 RED-HR locus rescue and engineering procedure; Primer design and plasmid constructs; AtSTM gap-repair construct; Targeting construct backbone; Preparation of electrocompetent EL250 cells; Transformation of BAC F24o1 and induction of recombinogenic function in EL250; AtSTM locus rescue from BAC F24o1 by gap-repair HR.
Category:Plant Biology Protocols: Arabidopsis Genetics Protocols
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Date Added:January 06, 2008 09:21:45 AM
Number Hits:4
 

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