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Isolation of Arabidopsis Nuclei and Measurement of Gene Transcription Rates Protocol - Details

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Protocols » Listing Details
ID:2538
Title: Isolation of Arabidopsis Nuclei and Measurement of Gene Transcription Rates Protocol - http://www.natureprotocols.com/2007/01/25/isolation_of_arabidopsis_nucle.php
Description:Protocol for the isolation of Arabidopsis nuclei and measurement of gene transcription rates using nuclear run-on assays. Plant materials are ground in hexylene glycol-based buffers and highly enriched nuclear fractions are obtained using Percoll density gradients. Standard and small-scale protocols are presented, along with a tested method for nuclear run-on assays. The entire process may be completed within 3 days.
Category:Plant Biology Protocols: Arabidopsis Genetics Protocols
Link Owner:
Date Added:January 06, 2008 09:18:28 AM
Number Hits:104
 

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