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EMS Mutagenesis of Arabidopsis Seed Protocol - Details

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Protocols » Listing Details
ID:2532
Title: EMS Mutagenesis of Arabidopsis Seed Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4621
Description:EMS is used at concentrations that induce multiple point mutations in each plant, such that mutant alleles of a specific locus are found at a rate of ~1 in 2000-5000 M2 plants. This high rate of mutagenesis makes possible the screening of relatively few plants to find those with the phenotype of interest, a particular advantage if the screen is laborious or if only a small number of genes mutate to the required phenotype.
Category:Plant Biology Protocols: Arabidopsis Genetics Protocols
Link Owner:
Date Added:January 06, 2008 09:02:38 AM
Number Hits:158
 

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