Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA Protocol - Details

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ID:	2516
Title:	Preparation of Uracil-containing Single-stranded Bacteriophage M13 DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3811
Description:	Single-stranded templates of bacteriophage M13 DNA containing 20-30 residues of uracil in place of thymine are generated during growth of the bacteriophage in an F' strain of E. coli carrying mutations in the ung and dut genes. This DNA is used as a template in the Kunkel method of oligonucleotide-directed mutagenesis (Oligonucleotide-directed Mutagenesis of Single-stranded DNA).
Category:	Viral Manipulation Protocols: M13 Phage Manipulation Protocols
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Date Added:	January 05, 2008 05:23:39 AM
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