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Large-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNA Protocol - Details

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Protocols » Listing Details
ID:2513
Title: Large-scale Preparation of Single-stranded and Double-stranded Bacteriophage M13 DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3284
Description:Protocol used chiefly to generate large stocks of double-stranded DNA of strains of M13 that are routinely used as cloning vectors. Large amounts of single-stranded DNA of an individual recombinant may occasionally be needed for specific purposes, e.g., to generate many preparations of a particular radiolabeled probe or to construct large numbers of site-directed mutants.
Category:Viral Manipulation Protocols: M13 Phage Manipulation Protocols
Link Owner:
Date Added:January 05, 2008 05:20:17 AM
Number Hits:65
 

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