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Cloning into Bacteriophage M13 Vectors Protocol - Details

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Protocols » Listing Details
ID:2512
Title: Cloning into Bacteriophage M13 Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4018
Description:Protocol describes three standard methods to construct bacteriophage M13 recombinants: (1) ligating insert DNA to a linearized vector, prepared by cleavage of M13 RF with a single restriction enzyme; (2) using alkaline phosphatase to suppress self-ligation of the linearized vector, and (3) using M13 RF cleaved with two restriction enzymes for directional cloning.
Category:Viral Manipulation Protocols: M13 Phage Manipulation Protocols
Link Owner:
Date Added:January 05, 2008 05:18:21 AM
Number Hits:142
 

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