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Preparation of Bacteriophage lambda DNA Cleaved with Two Restriction Enzymes Protocol - Details

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Protocols » Listing Details
ID:2509
Title: Preparation of Bacteriophage lambda DNA Cleaved with Two Restriction Enzymes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3987
Description:Many replacement vectors (e.g., the EMBL series, {lambda}2001, and {lambda}DASH) contain a series of restriction sites, arranged in opposite orientations, at each end of the central stuffer fragment. Digestion of these vectors with two different restriction enzymes yields left and right arms, a stuffer fragment, and short segments of the polycloning sites. These can easily be removed from the arms by differential precipitation with isopropanol or spun-column chromatography.
Category:Viral Manipulation Protocols: Phage Lamda Manipulation Protocols
Link Owner:
Date Added:January 05, 2008 05:13:59 AM
Number Hits:14
 

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