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Identifying DNA-binding Proteins in Bacteriophage ambda Expression Libraries Protocol - Details

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Protocols » Listing Details
ID:2504
Title: Identifying DNA-binding Proteins in Bacteriophage ambda Expression Libraries Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3785
Description:Protocol describes how to identify cloned cDNAs encoding proteins that bind to specific DNA sequences. The methods used are very similar to those used for immunological screening of expression libraries except that the nitrocellulose filters carrying immobilized proteins are screened with 32P-labeled double-stranded DNA rather than with antibodies.
Category:Viral Manipulation Protocols: Phage Lamda Manipulation Protocols
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Date Added:January 05, 2008 05:05:08 AM
Number Hits:9
 

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