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Alkaline Phosphatase Treatment of Bacteriophage lambda Vector DNA Protocol - Details

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Protocols » Listing Details
ID:2499
Title: Alkaline Phosphatase Treatment of Bacteriophage lambda Vector DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3976
Description:Removal of the 5'-phosphate groups from the internal termini of bacteriophage {lambda} arms is used to prevent self-ligation and suppress the background of nonrecombinant bacteriophages during cloning.
Category:Viral Manipulation Protocols: Phage Lamda Manipulation Protocols
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Date Added:January 05, 2008 04:02:42 AM
Number Hits:133
 

 

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