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Producing Single-stranded DNA with Phagemid Vectors Protocol - Details

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Protocols » Listing Details
ID:2495
Title: Producing Single-stranded DNA with Phagemid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4019
Description:Protocol describes methods to superinfect bacteria carrying a recombinant phagemid with a high-titer stock of an appropriate helper virus and to assay the yield of filamentous virus particles that carry single-stranded copies of the phagemid DNA. The key to success in using phagemids is to prepare a stock of helper virus whose titer is accurately known.
Category:Viral Manipulation Protocols: Phage Protocols
Link Owner:
Date Added:January 05, 2008 03:56:03 AM
Number Hits:34
 

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