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Screening Bacterial Colonies by Hybridization: Intermediate Numbers Protocol - Details

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Protocols » Listing Details
ID:2489
Title: Screening Bacterial Colonies by Hybridization: Intermediate Numbers Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3946
Description:Bacterial colonies growing on agar plates are transferred en masse to nitrocellulose filters. The spatial arrangement of colonies on the plates is preserved on the filters. After transfer, the filters are processed for hybridization to an appropriate radiolabeled probe while the original (master) plate is incubated for a few hours to allow the bacterial colonies to regrow in their original positions.
Category:Bacterial Protocols
Link Owner:
Date Added:January 04, 2008 05:41:25 AM
Number Hits:19
 

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