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Method to Analyze Meiotic Segregation Patterns in Coprinus cinereus Using PCR - Details

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Protocols » Listing Details
ID:2464
Title: Method to Analyze Meiotic Segregation Patterns in Coprinus cinereus Using PCR - http://www.fgsc.net/fgn/freedman.html
Description:Quick and reliable method to analyze meiotic segregation patterns in Coprinus cinereus using the polymerase chain reaction. The advantages of this method include: 1. The tissue is grown and lyophilized in the same tube, which facilitates the simultaneous analysis of many segregants. 2. Only one extraction step is necessary. 3. The markers are scored by gel electrophoresis, thereby bypassing Southern analysis.
Category:Fungi Protocols: Fungi Genetics Protocols
Link Owner:
Date Added:January 03, 2008 10:49:28 AM
Number Hits:7
 

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