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Protocol Guide for the N. crassa Yeast Artificial Chromosome Library - Details

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Protocols » Listing Details
ID:2355
Title: Protocol Guide for the N. crassa Yeast Artificial Chromosome Library - http://www.fgsc.net/fgn41/yac1.html
Description:Protocol guide for the N. crassa yeast artificial chromosome library. Includes: Chromosome Walking; Hybridization screening of the YAC library; YAC restriction mapping and contig building; Preparation of chromosomal DNA plugs of YAC clones; Partial restriction enzyme digestion of YAC DNA plugs; Using CHEF gel analysis to resolve YAC clones; Southern Hybridization; Isolation of terminal restriction fragments from cloned DNA inserts in YAC clones; etc.
Category:Fungi Protocols: Neurospora Protocols
Link Owner:
Date Added:December 28, 2007 12:37:12 AM
Number Hits:3
 

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