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Isolation of BAC DNA from Small-scale Cultures Protocol - Details

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Protocols » Listing Details
ID:2335
Title: Isolation of BAC DNA from Small-scale Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4006
Description:BAC DNAs are prepared from 5-ml cultures of BAC-transformed cells by a modification of the standard alkaline lysis method (Preparation of Plasmid DNA by Alkaline Lysis with SDS: Minipreparation). The yield typically varies between 0.1 and 0.4 µg of BAC DNA.
Category:BACs Bacterial Artificial Chromosome Protocols
Link Owner:
Date Added:December 26, 2007 10:42:13 PM
Number Hits:140
 

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