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BAC-FISH Protocol - Details

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Protocols » Listing Details
ID:2332
Title: BAC-FISH Protocol - http://www.nhm.ac.uk/hosted_sites/schisto/protocols/BAC_FISH.html
Description:Protocol uses the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labelled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other manufacturers may work equally well but have not been tested. Includes: Labeling of BAC clones; Ethanol precipitation; Hybridization; Post-hybridisation treatment / detection.
Category:BACs Bacterial Artificial Chromosome Protocols
Link Owner:
Date Added:December 26, 2007 10:39:15 PM
Number Hits:219
 

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