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Immunoprecipitation of mRNA-Protein Complexes Protocol - Details

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Protocols » Listing Details
ID:2314
Title: Immunoprecipitation of mRNA-Protein Complexes Protocol - http://www.nature.com/nprot/journal/v1/n2/pdf/nprot.2006.82.pdf
Description:Protocol for immunoprecipitation of mRNA-protein complexes. In this protocol, an antibody targeting an RBP of interest is used to immunoprecipitate the RBP and any interacting molecules from a cell lysate. Reverse transcription followed by PCR is then used to identify individual mRNAs isolated with the RBP. This method focuses on examining an association between a specific RBP-mRNA complex, and it is best suited for a small scale screening of known or putative binding partners.
Category:Immunology: Immunoprecipitation Protocols
Link Owner:
Date Added:December 22, 2007 08:31:54 AM
Number Hits:65
 

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