Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Epitope Tagging of Recombinant Proteins Protocol - Details

Link Details 

Protocols » Listing Details
ID:2313
Title: Epitope Tagging of Recombinant Proteins Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E663998FCCB61070F32F1EAE0301F7F&objectid=6677C749FA75FD435618AC07256968A7
Description:Protocols for detection and purification of proteins tagged with a particular epitope, the FLAG tag, although the same general approach can be applied to other epitope tags. The protocols employ the anti-FLAG M2 antibody to detect and purify FLAG-tagged proteins. The methods presented are immunoprecipitation of FLAG fusion proteins from cells using an anti-FLAG M2 affinity gel, detection of FLAG fusion proteins by western blotting, and purification of FLAG fusion proteins by anti-FLAG.
Category:Immunology: Immunoprecipitation Protocols
Link Owner:
Date Added:December 22, 2007 08:29:15 AM
Number Hits:247
 

Protocols Tags

  • adherent Protocols
  • affinity Protocols
  • anti Protocols
  • assay Protocols
  • cdc42 Protocols
  • cdna Protocols
  • cell Protocols
  • cells Protocols
  • choosing Protocols
  • components Protocols
  • culture Protocols
  • cytoskeletal Protocols
  • derivation Protocols
  • detection Protocols
  • dna Protocols
  • exposure Protocols
  • extract Protocols
  • formal Protocols
  • fusion Protocols
  • gel Protocols
  • glutathione Protocols
  • hoechst Protocols
  • how Protocols
  • hybridization Protocols
  • hybrids Protocols
  • imaging Protocols
  • includes Protocols
  • information Protocols
  • lines Protocols
  • melanin Protocols
  • methods Protocols
  • microscopy Protocols
  • molecular Protocols
  • plant Protocols
  • precipitation Protocols
  • prime Protocols
  • protein Protocols
  • proteins Protocols
  • provides Protocols
  • quantitative Protocols
  • rac Protocols
  • ras Protocols
  • rho Protocols
  • rna Protocols
  • should Protocols
  • stain Protocols
  • staining Protocols
  • stem Protocols
  • that Protocols
  • viability Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum