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Using FLAG Epitope-Tagged Proteins for Coimmunoprecipitation of Interacting Proteins Protocol - Details

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Protocols » Listing Details
ID:2308
Title: Using FLAG Epitope-Tagged Proteins for Coimmunoprecipitation of Interacting Proteins Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/28/pdb.prot4557
Description:Protocol describes the use of FLAG-epitope-tagged proteins for both small-scale analysis and large-scale coimmunoprecipitation of interacting proteins. When examining protein interactions, it is sometimes possible to immunoprecipitate an endogenous protein X directly, without using an epitope tag, if antibodies are available. The advantage of examining interactions of endogenously expressed proteins is that these are likely to be physiological and less likely to be an artifact of overexpression.
Category:Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols
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Date Added:December 22, 2007 08:12:19 AM
Number Hits:73
 

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