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Nondenaturing Protein Immunoprecipitation from Mammalian Cells Protocol - Details

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Protocols » Listing Details
ID:2307
Title: Nondenaturing Protein Immunoprecipitation from Mammalian Cells Protocol - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4620
Description:Protocol describes a nondenaturing immunoprecipitation (IP) for mammalian cells. Prefer to use denaturing IPs to recover labeled proteins from pulse-chase experiments. However, the nondenaturing protocol is useful when one wishes to separate soluble from insoluble proteins, or when the antibody being used recognizes a native epitope.
Category:Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols
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Date Added:December 22, 2007 08:10:43 AM
Number Hits:69
 

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