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Immunoprecipitation: Preclearing the Lysate Protocol - Details

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Protocols » Listing Details
ID:2306
Title: Immunoprecipitation: Preclearing the Lysate Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4535
Description:To reduce backgrounds and to improve the signal-to-noise ratio, an antibody that does not recognize the antigen being studied can be added to the lysate and processed as for a normal immunoprecipitation. Any nonspecific proteins that might contaminate the final immunoprecipitation step are presumably removed with this irrelevant antibody.
Category:Immunology: Immunoprecipitation Protocols: Protein Immunoprecipitation Protocols
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Date Added:December 22, 2007 08:09:19 AM
Number Hits:223
 

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