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Metabolic Labeling & Immunoprecipitation Protocols - Details

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Protocols » Listing Details
ID:2301
Title: Metabolic Labeling & Immunoprecipitation Protocols - http://www.cbrinstitute.org/labs/springer/protocols/jun_35Slabeling_IP.html
Description:Cells incorporate 35S-methionine or cysteine during the protein synthesis. Thus it is essential to use Met,Cys-free medium and dialyzed FCS during the labeling. Short period of incubation with 35S-methionine or cysteine will result in radiolabeling (pulse), and additional incubation with excess concentration of unlabeled Met+Cys (chase) is needed for complex glycoproteins like integrins to get expressed as a maturated form.
Category:Immunology: Immunoprecipitation Protocols: Immunoprecipitation of Radiolabeled Cells Protocols
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Date Added:December 22, 2007 07:58:11 AM
Number Hits:20
 

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